AHP1900 specifically recognises an epitope within the N-terminal (NT) region of Adiponectin Receptor protein 2 (AdipoR2), a transmembrane protein and member of the ADIPOR family, expressed mainly in the liver and skeletal muscle, which acts a receptor for both globular and full-length adiponectin.
AdipoR2 acts as a mediator of fatty acid oxidation, glucose uptake by adiponectin and increased AMPK, PPARalpha ligand activity, thereby playing an important role in the regulation of glucose and lipid metabolism, and in oxidative stress.
AHP1900 is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
1/250 -
Immunoprecipitation
Western Blotting
1/1000 -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human AdipoR2 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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