AHP1898 specifically recognises human PI3-kinase p110 subunit alpha (Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), otherwise known as PIK3CA, a member of the PI3/PI4-kinase family of lipid kinases, implicated in the regulation of signal transduction during cell growth.
PI3-kinase is a heterodimer consisting of a p85 regulatory subunit and a p110 catalytic subunit, which itself is divided into isoforms p110alpha, p110beta, p110delta and p110gamma. Studies have shown that defects in p110alpha are associated with breast, colorectal and ovarian cancers.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/128000 -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
2.0 - 3.0ug/ml
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human p110alpha was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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