RABBIT ANTI RAT GABA A RECEPTOR GAMMA 2 (pSer327)

产品编号：AHP1877 品牌：AbD Serotec 原厂货号：AHP1877
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

0.1 ml

运保温度:

+4oC or -20oC

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描述：

Specificity

AHP1877 is specific for the gamma 2 subunit of rat gamma-aminobutyric acid A (GABA_A) receptor, when phosphorylated on Ser327.

GABA_A receptors are GABA gated chloride channels, which are important mediators of neuronal inhibition in the mammalian central nervous system. Activation of the GABA_A site results in increased intracellular chloride concentration and hyperpolarization of the cell.

GABA_A receptors are pentameric structures assembled from a family of distinct subunits. At least fourteen distinct subunits have been identified, which show distinct patterns of temporal and spatial expression.

GABA_A receptors may be modulated by several drug types including benzodiazepines and barbiturates, and by phosphorylation by protein kinase C (PKC) and protein kinase A (PKA).

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine	Expected from Sequence
Chicken	Expected from Sequence
Dog	Expected from Sequence
Human	Expected from Sequence
Mouse	Expected from Sequence
Primate	Expected from Sequence

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting⁽¹⁾				1/1000

(1)

For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting

AHP1877 detects a band of approximately 45kDa in rat cortex.

Storage

Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Antiserum Preparation

Antisera to rat GABA_A receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.