AHP1859 recognises human matrix metalloproteinase 1 (MMP-1). The MMP’s are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP’s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-1 is one of the collagenases, capable of degrading collagens I, II and III, all main components of the interstitial stroma. It is overexpressed in invasive melanoma, colorectal and esophageal cancers. It has also been implicated in arthritis and might influence atherosclerotic lesion formation. It might also be involved in the repair processes of the heart after myocardial infarction.
Species Cross-Reactivity
Target Species
Cross Reactivity
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
5ug/ml -
Western Blotting
1 - 2ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to MMP-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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