AHP1828 specifically recognises human Elongin A (EloA), otherwise known as transcription elongation factor B polypeptide 3, a general transcription elongation factor, which stimulates RNA polymerase II transcription elongation beyond template-encoded arresting sites.
Elongin exists as a heterotrimer consisting of an A (A1, A2 or A3), B and C subunit. Subunit A is transcriptionally active, with activity strongly enhanced by binding to the dimeric complex of the elongin regulatory subunits B and C (elongin BC complex), which is mediated by subunit C.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human Elongin A was raised by repeated immunisation of chickens with highly purified antigen.