AHP1825 recognises Erythropoietin Receptor (EPOR), a type I transmembrane protein and member of the type I cytokine receptor family, expressed by erythroid cells and erythroid progenitor cells, which acts as a receptor for erythropoietin (EPO) to mediate erythroblast proliferation and differentiation, and maintain progenitor survival.
Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3. Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.
AHP1825 has been shown to block Iodine125labelled erythropoietin ligand binding using erythroleukemia cell line TF-1 with 3000 EPOR/cell in neutralization studies, seeHarris, K.W.et al.for details.
AHP1825 is reported as suitable for use in immunocytochemistry.
AHP1825 has been reported for use in functional studies. Removal of Sodium Azide is recommended prior to use in functional assays. AbD Serotec recommend the use ofEQU003for this purpose. The recommended concentration for functional assays is 500ug/ml.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
50ng/ml -
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
10ug/ml -
Western Blotting
10ug/ml -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1825 detects a 49kDa band of EPOR-GST on SDS-PAGE.
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human EPOR was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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