AHP1814 specifically recognises human Toll-like receptor 5 (TLR5), a member of the evolutionarily conserved Toll-like receptor family, highly expressed by peripheral blood leukocytes, and in particular monocytes, which signals through the adaptor proteins MyD88 and TRAF6, inducing the activation of the transcription factor NF-kappaB.
TLR5 plays an important role in the innate immune response to pathogenic bacteria, through the recognition of the protein monomer flagellin, which upregulates TLR5 expression.
AHP1814 is reported as suitable for use in immunocytochemistry.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/75000
Flow Cytometry
1/200
Immunohistology - Frozen
Immunohistology - Paraffin
1/250
Immunoprecipitation
Western Blotting
1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106cells in 100ul.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human TLRS was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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