AHP1811 specifically recognises an epitope within the N-terminal (NT) region of human CDw198, otherwise known as CCR8 (C-C chemokine receptor type 8), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which acts a high-affinity receptor for the chemokine I-309 (CCL1), implicated in the regulation of monocyte chemotaxis and thymocyte apoptosis.
CDw198 is constitutively expressed by monocytes, preferentially expressed on Th2 (type 2 helper T) cells, and acts as a co-receptor with CD4 for HIV1 infection.
AHP1811 is a neutralizing antibody which inhibits Lipoprotein(a) induced monocyte chemotactic assay in HUVECs. AHP1811 is reported as suitable for use in immunocytochemistry on myelogenous leukaemia K562 cells.
Removal of Sodium Azide is recommended prior to use in functional assays. AbD Serotec recommend the use ofEQU003for this purpose.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/100000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
1/250
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CDw198 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.