Rabbit anti Human defensin beta-1 polyclonal antibody recognises human defensin beta 1 (BD-1), a secreted cationic antimicrobial peptide and member of the beta-defensin family, expressed at epithelial surfaces, which plays an important role in innate immunity.
Studies byGoldmanet al.have shown that the activity of BD-1 is compromised in patients with Cystic Fibrosis due to salt-dependent inactivation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human defensin beta 1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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