AHP1786 recognises Bruton tyrosine kinase (BTK), also known as Agammaglobulinaemia tyrosine kinase. BTK was initially identified as playing a key role in the primary immunodeficiency disease X-linked agammaglobulinaemia (XLA). XLA is characterised by defects in the maturation pathways of B cells, with virtually no mature circulating B cells. Patients suffer from lack of immunoglobulins of all classes and resulting recurrent infection. BTK is crucial for B cell maturation and transiently phosphorylates General transcription factor II-I (BTK-associated protein 135) in response to B cell receptor stimulation. BTK also interacts with members of the Toll-like receptor family and proteins involved in the TLR4 signal transductions such as MyD88, TIRAP and IRAK.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
10ug/ml
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1786 detects a band of approximately 80kDa in U937 cell lysate
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to BTK (NT) was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
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