AHP1775 recognised human Neuronal Apoptosis Inhibitory Protein (NAIP) also known as BIRC1, a 1406 amino acid intracellular protein belonging to the IAP family.
NAIP functions in the prevention of neuron apoptosis induced by various signals. It was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. NAIP, unlike other IAPs, requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, this suggests that NAIP is unique among the IAPs in its regulation of activity.
NAIP is expressed in motor, but not sensory neurons, it is also found in the liver, placenta, and to a lesser extent, spinal cord.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
5ug/ml
Western Blotting
0.5 - 1ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human Neuronal Apoptosis Inhibitory Protein were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
