AHP1745B specifically recognises human FGF 10 (fibroblast growth factor 10), a member of the heparin-binding growth factors family which act as promoters of cellular differentiation and proliferation, and therefore play key roles in embryonic development, morphogenesis, tissue regeneration and tumour growth and invasion.
FGF 10 signals through binding to the FGF Receptor (FGFR) 2b, and has structural and biological activities similar to that of FGF 7. Defects in FGF 10 are responsible for the autosomal dominant disorders known as lacrimo-auriculo-dento-digital syndrome (LADDS), and aplasia of lacrimal and salivary glands (ALSG).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human FGF 10 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.