AHP1744B specifically recognises human FGF 4 (fibroblast growth factor 4), a member of the heparin-binding growth factors family which act as promoters of cellular differentiation and proliferation, and therefore play key roles in embryonic development, morphogenesis, tissue regeneration and tumour growth and invasion.
FGF 4 was originally designated as hst, which was identified as a transforming gene in DNA samples from human stomach cancer, and shown to be secreted by both embryonic stem cells and embryonic carcinoma. Studies have shown that FGF 4 signals through binding to the FGF Receptors (FGFRs) 1c, 2c, 3c, and 4.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human FGF 4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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