AHP1741 detects an epitope within the internal region of human CD49d, a 150kD protein also known as integrin alpha 4 (ITGA4). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. CD49d associates with CD29 (ITGB1) and ITGB7, to form VLA-4 (very late antigen-4), and a4ß7 integrin respectively.
VLA-4 is expressed on lymphocytes, monocytes, mast cells, macrophages, basophils and eosinophils, and has key roles in inflammation, cellular adhesion and cellular activation. a4ß7 is expressed mainly on lymphocytes, and plays a key role in lymphocyte recruitment to sites of inflammation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Paraffin(1)
10ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human CD49d were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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