AHP1734 recognises human Transforming Growth Factor Beta 1 (TGF-beta1), a 390 amino acid secreted protein belonging to the TGF-beta family.
TGF-beta1 is a multifunctional protein that controls proliferation, differentiation and other functions in a wide range of cell types and both positively and negatively regulates many other growth factors.
TGF-beta1 also plays an important role in bone remodelling through stimulation of oesteoblastic bone formation causing chemotaxis, proliferation and differentiation in committed osteoblasts.
TGFB1 is synthesized by many cells and have specific receptors for it, and it is highly expressed in bone.
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CED), also known as progressive diaphyseal dysplasia 1 (DPD1).
Species Cross-Reactivity
Target Species
Cross Reactivity
Mammals
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/2000 - 1/10000
Immunohistology - Paraffin(1)
5ug/ml
Western Blotting
1/200 - 1/1000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Human TGF-beta1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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