AHP1729 recognizes Mouse RAS-responsive element binding protein 1 (RREB1), a 185kDa nuclear protein belonging to the Krueppel C2H2-type zinc-finger protein family.
RREB1 is a transcription factor that binds specifically to the distal RAS-responsive element (RRE) in the calcitonin gene promoter and augments the Ras/Raf-mediated transcriptional response of that promoter. Research indicates that RREB1 may be involved in Ras/Raf-mediated cell differentiation. RREB is expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas but is absent from the brain
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/2000 - 1/10000
Immunohistology - Paraffin(1)
10ug/ml
Western Blotting
1/5000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to mouse RREB1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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