AHP1679 specifically recognises human KCNJ11, the gene which encodes the Kir6.2 subunit of the ATP-sensitive K+channel, an integral membrane voltage-gated channel and member of the inward rectifier-type potassium channel family, which favours the flow of potassium (K+) into, rather than out of, a cell.
Defects in the KCNJ11 gene are responsible for the rare insulin-requiring hyperglycaemia conditions known as permanent neonatal diabetes mellitus (PNDM), and transient neonatal diabetes mellitus type 3 (TNDM3). Defects in KCNJ11 are also responsible for familial hyperinsulinemic hypoglycaemia type 2 (HHF2), the most common cause of persistent hypoglycaemia during infancy.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.3ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1679 detects a band of approximately 45-48kDa in human muscle cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human KCNJ11 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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