AHP1677 specifically recognises human CD344, otherwise known as Frizzled-4 (FZD4), a seven-transmembrane domain protein and member of the G-protein coupled receptor Fz/Smo family, which acts as a receptor for Wnt proteins in the activation of the Wnt/ß-catenin pathway.
There is evidence that CD344 may play a role in retinal angiogenesis, since defects in CD344 are responsible for the familial retinal disease known as exudative vitreoretinopathy 1 (FEVR), and also the cause of the retinal neovascularization disease, Criswick-Schepens syndrome. CD344 has been shown to be a marker for neuronal stem cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/16000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.3 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1677 detects a band of approximately 60kDa in human colon and ileum cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CD344 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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