AHP1675 specifically recognises human DNA (cytosine-5)-methyltransferase 1, also known as DNMT1, a ubiquitously expressed nuclear enzyme and member of the C5-methyltransferase family, which plays a key role in the maintenance of DNA cytosine methylation patterns, and therefore in the regulation of gene expression, X chromosome inactivation, genomic imprinting and development.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Dog
Expected from Sequence
Mouse
Expected from Sequence
Pig
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1675 detects a band of approximately 170kDa in Jurkat cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to DNMT1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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