AHP1670 recognises the enzyme Arylsulfatase A (ARSA), otherwise known as cerebroside-sulfatase, a member of the sulfatase family which hydrolyzes the conversion of cerebroside sulfate to cerebroside and sulphate.
A deficiency in ARSA levels is responsible for the lysosomal storage disease known as Metachromatic leukodystrophy (MLD), a fatal autosomal recessive trait characterized by an intralysosomal accumulation of cerebroside-3-sulfate, which also effects myelin membranes, resulting in progressive demyelination.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/32000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1670 detects a band of approximately 55kDa in mouse testis cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to ARSA was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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