AHP1669 specifically recognises human ASS1 (Argininosuccinate synthetase 1), an ornithine (urea) cycle enzyme and member of the argininosuccinate synthase family, which catalyzes the penultimate step of the arginine biosynthetic pathway.
Defects in ASS1 genes are responsible for the autosomal recessive disease known as citrullinemia type 1 (CTLN1), characterized by increased levels of urine and serum citrulline, and ammonia intoxication.
AHP1669 is reported as suitable for use in immunoprecipitation studies.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Mouse
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/64000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.01 - 0.03ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1669 detects a band of approximately 45kDa in human kidney cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to ASS1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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