AHP1668 specifically recognises an epitope within the N-terminal (NT) region of human Beta-3 adrenergic receptor, otherwise known as ADRB3. ADRB3 is a transmembrane protein and member of the G-protein coupled receptor 1 family primarily expressed by adipose tissue, which acts as a regulator of lipolysis and heat production (thermogenesis).
The ADRB3 variant Arg-64 has been identified as a possible candidate gene linked with obesity, and to an increased susceptibility to non-insulin-dependent diabetes mellitus (NIDDM).
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/128000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.03 - 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1668 detects a band of approximately 45kDa in human brain cell lysates
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to ADRB3 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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