RABBIT ANTI CARD11

产品编号：AHP1665T 品牌：AbD Serotec 原厂货号：AHP1665T
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

50 μg

运保温度:

+4oC or -20oC

到货周期：

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描述：

Specificity

AHP1665T recognises Caspase recruitment domain family member 11 (CARD11, also known as CARMA1), a member of the membrane-associated guanylate kinase-like (MAGUK) family. These proteins act as molecular scaffolds assisting in the assembly of signal transduction molecules. CARD11 phosphorylates BCL10 and induces NF-kappa-B activity via BCL10 and IKK.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Yes

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
Immunohistology - Paraffin⁽¹⁾				10ug/ml
Western Blotting				0.5 - 2.0ug/ml

(1)

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting

AHP1665T detects a band of approximately 125kDa in human thymus cell lysate

Storage

Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Antiserum Preparation

Antiserum to CARD11 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.