AHP1660 recognises DEDD2 (DNA-binding death effector domain-containing protein 2), expressed in most tissues, particularly liver, kidney, heart, ovary, spleen, testes, skeletal muscle and peripheral blood leukocytes. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus when apoptosis is induced. DEDD2 may target caspase-8 and -10 to the nucleus, thus playing an important role in death receptor-induced apoptosis. It might also play a role in the regulation of the degradation of intermediate filaments during apoptosis. DEDD2 also interacts with FLIP.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1660 detects a band of approximately 26kDa in RAW264.7 cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to DEDD2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
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