AHP1657T recognises human Nudel, or endooligopeptidase A, a protein highly expressed in the brain where it associates with dynein motors and Lis1, both of which have been linked to neuronal development and migration. Nudel facilitates the polymerisation of neurofilaments and the organisation of the cellular microtubule array and microtubule anchoring at the centrosome. It is required for several dynein- and microtubule-dependent processes such as the centripetal motion of secretory vesicles, coupling of the nucleus and centrosome and maintaining Golgi integrity. Nudel also serves as a Cdk5 substrate and its phosphorylation status affects its localisation in neurons.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
2ug/ml
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1657T detects a band of approximately 33kDa in Jurkat cell lysate
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to Nudel was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
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