AHP1652T recognises human Autophagy-related protein 7, known also as Ubitiquin Activating Enzyme-E1 like Protein, ATG7 and APG7, a 78kDa cytoplasmic protein.
In mammalian cells, ATG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death.
ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
10ug/ml
Western Blotting
1 - 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1652T detects a band of approximately 78kDa in L1210 cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human ATG7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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