AHP1610 recognises human NF-kB-activating kinase (NAK), otherwise known as TANK-binding kinase 1 (TBK1). It is involved in the signalling cascade of NF-kB activation by directly phosphorylating and activating IKK Beta. It also mediates the production of IFN Beta and CCL5 and plays a key role in the innate immune response. In addition, it phosphorylates and activates the interferon regulatory factors -3 and -7, eventually leading to the development of a cellular antiviral state. It may also be involved in the induction of the antiviral interferon response. NAK is expressed ubiquitously, with higher expression in testis.
AHP1610 does not cross-react with IKK Alpha, IKK Beta, IKK Gamma or IKK Epsilon.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
10ug/ml
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1610 detects a band of approximately 84kDa in MOLT4 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human NAK was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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