AHP1607T recognises human Inhibitor of nuclear factor kappa-B (NF-kB) kinase subunit gamma (IKK gamma). IKK gamma is the regulatory subunit of the IKK core complex which phosphorylates the inhibitors of NF-kB, thereby leading to the dissociation of the inhibitor/NF-kB complex and eventually the degradation of the inhibitor. IKK gamma is also a mediator for TAX activation of NF-kB and might be implicated in the NF-kB mediated protection from cytokine toxicity. It is found in the heart, lung, liver, skeletal muscle, kidney and pancreas.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1607T detects a band of approximately 52kDa in HeLa cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human IKK Gamma were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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