AHP1600 recognises the extracellular (N-terminal) domain of human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1 or Flt-1), a 180 kDa membrane receptor tyrosine kinase. It is expressed by almost all vascular endothelial cell and some peripheral blood monocytes. Vascular Endothelial Growth Factor is a regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions.
Species Cross-Reactivity
Target Species
Cross Reactivity
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/500 - 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1600 detects a band of approximately 75kDa in human endothelial cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human VEGF receptor 1 were raised by repeated immunisations of rabbits with highly purified antigen.
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