AHP1599 recognises human FTO, otherwise known as fat mass and obesity-associated protein, a 60kDa protein expressed ubiquitously with particularly high levels in the adrenal glands and brain. The gene was named FTO because a deletion in it resulted in fused toes and other abnormalities in mice. Since then, some correlation has been shown between carriers of the gene and obesity as well as an increased risk of Type 2 diabetes.
The precise function of the gene is unknown but it shows homology with the enzyme AlkB, which oxidatively demethylates DNA. FTO gene expression has been shown to be highly upregulated in rats after food deprivation. A negative correlation with the expression of orexin peptide, which is involved in the stimulation of food intake, has also been found.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/500 - 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1599 detects a band of approximately 60kDa in epithelial cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human FTO were raised by repeated immunisations of rabbits with highly purified antigen.