AHP1587 recognises Human G-protein coupled receptor 15 (GPR15) also known as Brother of Bonzo (BOB), a 40.8kDa multi-pass membrane protein.
Human immunodeficiency virus (HIV) and related viruses require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four additional human molecules GPR15, STRL33, GPR1 and V28 have now been identified as HIV coreceptors. The genes encoding human and monkey GPR15/BOB have been cloned and research has demonstrated that his novel G protein-coupled receptor serves as co-receptor for simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
0.5 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1587 detects a band of approximately 50kDa in human spleen cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human GRP15 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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