AHP1584 specifically recognises MEK 1 and MEK 2, also known as MAPKK1 and MAPKK2 respectively, related dual specificity protein kinases and members of the STE serine/threonine protein kinase family, which play a critical role in the evolutionarily conserved MAP kinase signalling cascade controlling cell growth and differentiation.
MEK 1 and MEK 2 are activated through the phosphorylation of serine residues Ser218 and Ser222 by Raf, which then act together to phosphorylate ERK (mitogen-activated protein kinase) on a threonine and tyrosine residue. The activated ERK then moves to the nucleus where it activates transcription factors involved in the regulation of gene expression.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mammals
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1584 detects a band of approximately 45kDa in RAJI human Burkitt Lymphoma whole cell lysates under reducing conditions.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human MEK1/2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by immunoaffinity chromatography.
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