AHP1578 specifically recognises an epitope within the C-terminal (CT) region of RAB11A (ras-related protein Rab-11A), a ubiquitously expressed GTP-binding protein and member of the small GTPase superfamily, involved in the endosomal recycling of internalized receptors, and the trafficking of proteins from the Golgi apparatus to the plasma membrane.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Monkey
Expected from Sequence
Mouse
Yes
Rabbit
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1578 detects a band of approximately 25-28kDa in mouse heart cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human RAB11A (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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