AHP1575 specifically recognises an epitope within the C-terminal (CT) region of human p70S6K, otherwise known as RPS6KB1 (Ribosomal protein S6 kinase beta-1), a serine/threonine kinase which acts upon 40S ribosomal protein S6 to induce protein synthesis, allowing for efficient G1 phase progression.
p70S6K is activated following phosphorylation of several sites, in response to hormones, growth factors, mitogenic stimuli and physical exercise, and is a downstream effector in the AKT/mTOR (mammalian target of rapamycin) signalling pathway. Studies have implicated that as well as its role in translation, p70S6K acts as a regulator of cell motility, invasion, and cytoskeletal reorganization.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Rabbit
Expected from Sequence
Rat
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/128000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.2 - 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1575 detects a band of approximately 65-70kDa in human muscle and placenta cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human p70S6K (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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