AHP1572 specifically recognises an epitope within the C-terminal (CT) region of isoform 2 of human IRSp53 (insulin receptor substrate p53), otherwise known as BAIAP2, a brain-enriched scaffolding protein which plays a key role in actin cytoskeletal reorganization and filopodia formation, acting as a signal transducing adaptor between membrane-bound Rho family GTPases Rho, Rac, and cdc42, and Rho family effectors mDia, WAVE2 and Mena.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/1000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.2 - 0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1572 detects a band of approximately 60kDa in human placenta cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human IRSp53 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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