AHP1550 recognises rat metabotropic glutamate receptor 5 and 1a (mGluR5 and mGluR1a). The metabotropic glutamate receptors have been organised into three groups, mGluR5 and mGluR1 make up group I which, when activated, stimulates phospholipase C and activates protein kinase C to hydrolyse phosphoinositide phospholipids in the cell membrane. Group I mGluRs, but not other groups, are activated by 3, 5-dihydroxyphenylglycine (DHPG).
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Expected from Sequence
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/500
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1550 detects bands of approximately 125kDa (monomer) and 250kDa (dimer) in HEK293 cells.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to metabotropic glutamate receptor 5/1a were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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