AHP1546 recognises extracellular loop 2 of the human dopamine transporter (DAT). DAT is a Na+/Cl-dependent transport protein that is responsible for clearing synaptic dopamine by transporting it into neurons. The protein is implicated in a number of dopamine-related disorders such as attention deficit hyperactivity disorder, Parkinson’s disease, depression and drug abuse. DAT consists of 12 transmembrane domains connected by intra- and extracellular loops. The largest, extracellular loop 2, occurs between transmembrane domains 3 and 4 and contains N-linked carbohydrates and a putative disulfide bond. It is the site on the DAT protein that is most sensitive to proteolysis.
Species Cross-Reactivity
Target Species
Cross Reactivity
Monkey
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/1000
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human dopamine transporter (Extracellular loop 2) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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