AHP1543 recognises rat 14-3-3 protein, a highly conserved, ubiquitously expressed protein family. 14-3-3 proteins form homo- and heterodimers that can interact with a variety of cellular proteins that are typically phosphorylated on serine or threonine residues. 14-3-3 proteins regulate cellular processes such as trafficking, cell-cycle regulatory pathways and signal transduction. Brain tissue contains the highest concentration of 14-3-3 proteins and the proteins have been found in the cerebral spinal fluid of patients with certain neurodegenerative disease such as Creutzfeldt-Jakob disease and scrapie. 14-3-3 proteins might also contribute to Alzheimer’s and Parkinson’s diseases, Miller-Dieker syndrome and spinocerebellar ataxia type 1. 14-3-3 has also been implicated in cancer.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chicken
Expected from Sequence
Dog
Expected from Sequence
Human
Expected from Sequence
Mouse
Expected from Sequence
Primate
Expected from Sequence
Xenopus
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1543 detects a band of approximately 29kDa in rat hippocampal lysate.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to 14-3-3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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