AHP1496 recognises human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family.
Antigen stimulation of immune cells triggers Ca2+entry through Ca2+release-activated Ca2+(CRAC) channels. CRAC channels are the main pathway for Ca2+influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.
ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.
AHP1496 is not expected to cross react with either ORAI1 or ORAI2.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
10ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1 - 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1496 detects a band of approximately 35kDa in A20 cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human ORAI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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