AHP1489 recognises human conserved dopamine neurotrophic factor (CDNF), a 20.9 kDa secreted protein belonging to the ARMET family.
CDNF is a neutrophic factor for dopaminergic neurons. Studies have demonstrated that CDNF can prevent the 6-hydroxylamine (6-OHDA)-induced degeneration of dopaminergic neurons in a rat model of Parkinson’s disease. Additionally CDNF has been shown to restore the dopaminergic function and prevent the degeneration of dopaminergic neurones in the substantia nigra, suggesting that CDNF may have potential in the treatment of Parkinson’s disease.
CDNF exists in at least two known isoforms and is widely expressed in both neuronal and non-neuronal tissues. Within the brain, highest expression is observed in the optic nerve and corpus callosum.
AHP1489 does not cross-react with mesencephalic-astrocyte-derived neuro-trophic factor (MANF).
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
Immunoprecipitation
Western Blotting
2 - 4ug/ml
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1489 detects a band of approximately 19kDa in mouse brain tissue cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CDNF was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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