AHP1481B specifically recognises human Galectin-3 (Gal-3), a member of the galectin family of beta-galactoside-binding proteins, which is involved in both intracellular and extracellular functions and exhibits anti-apoptotic properties.
Gal-3 is implicated in many biological processes, including cell differentiation, growth, adhesion, angiogenesis and inflammation, and is expressed in a wide range of neoplasms. Gal-3 acts as a regulator of tumour metastasis, and several studies have shown a correlation between levels of Gal-3 and tumour progression in liver, thyroid, colon, gastric and breast carcinomas, making Gal-3 an emerging cancer marker.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
0.25 - 1.0ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.1 - 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1481B may be used under either reducing or non-reducing conditions withPHP188as the positive control.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Antiserum Preparation
Antiserum to human galectin-3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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