AHP1474 specifically recognises human CoREST (REST corepressor 1), a ubiquitously expressed member of the CoREST family and component of the BHC (BRAF–HDAC) transcriptional corepressor complex, involved in the repression of neuronal-specific gene transcription.
The regulation of neuronal cell specific gene expression, is dependent on the repression of non-neuronal cell gene transcription, mediated by the neuronal silencer protein REST/NRSF (RE1 silencing transcription factor/neural-restrictive silencing factor) through recruitment of the BHC complex. CoREST is a SANT-domain-containing protein within the BHC complex, which mediates the nucleosomal demethylation of H3K4 (histone H3 lysine 4), and acts as a regulator of BHC corepressor function.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Western Blotting
1/2500 - 1/15000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1474 detects a band of approximately 70kDa in HeLa cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human CoREST was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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