AHP1473 specifically recognises human claspin, a cell cycle regulated adaptor protein which peaks at the S/G2phase and plays an essential role during checkpoint-mediated cell cycle arrest, in response to DNA damage or inhibition of DNA replication.
Following replication stress, the phosphorylation of threonine 916 and serine residues 945/982 on claspin, enables its association with Chk1 (cell cycle checkpoint kinasomologue 1), a protein kinase responsible for the initiation of G2arrest in response to DNA damage. Claspin has also been shown to interact with ATR (the ATM- and Rad3-related protein kinase) and Rad9, further checkpoint proteins involved in Chk1 activation.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
1.0 - 5.0ug/mg of lysate
Western Blotting
1/5000 - 1/20000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1473 detects a band of approximately 175kDa in HeLa cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human claspin was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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