AHP1465 recognises Mothers against decapentaplegic homolog 3 (SMAD3), a 50kDa transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase,
SMAD3 functions in cell proliferation, differentiation and development. Upon stimulation, SMAD3 oligomerizes with SMAD4 and translocates into the nucleus where it binds to DNA and transcription factors. SMAD3 activity induces differentiation of selected neuronal subtypes at the expense of other subtypes.
Studies have indicated that defects in SMAD3 may be a cause of colorectal cancer.
Species Cross-Reactivity
Target Species
Cross Reactivity
Amphibia
Yes
Bovine
Yes
Chicken
Yes
Mouse
Yes
Pig
Yes
Rat
Yes
Zebrafish
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/25000 - 1/100000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
2.5ug/ml
Western Blotting
1/2000 - 1/20000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human SMAD3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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