AHP1459 recognizes mouse Peroxisome proliferator-activated receptor alpha (PPAR ALPHA), a 52.2kDa transcription factor belonging to the nuclear hormone receptor (NR1) family.
Peroxisome proliferator-activated receptors are vital in the regulation of cellular differentiation, development and metabolism. PPARA is also the target of hypolipidemic fibrates, a class of drugs used in cholesterol disorders.
PPARA alpha is expressed primarily in the liver, kidney, heart and skeletal muscle and is involved in the regulation of beta-oxidation of fatty acids.
Species Cross-Reactivity
Target Species
Cross Reactivity
Chimpanzee
Yes
Human
Yes
Monkey
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/8000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
20ug/ml
Western Blotting
1/500
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to mouse PPAR ALPHA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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