AHP1455 recognizes the Mouse Zinc Finger protein 2 (GLI2), also known as GLI-Kruppel family member 2, a 133.3kDa protein belonging to the C2H2-type zinc finger protein subclass of the Gli family of transcription factors.
GLI2 and the related GLI3 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI2 being primarily a transcriptional activator while GLI3 is a transcriptional repressor. Studies indicate that GLI2 plays a role in embryogenesis. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.
Species Cross-Reactivity
Target Species
Cross Reactivity
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/15000 - 1/60000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
5ug/ml
Immunoprecipitation
Western Blotting
1/500 - 1/2000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to mouse GLI2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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