AHP1444 recognises Lissencephaly 1 (LIS1), also known as Platelet-Activating Factor Acetylhydrolase Isoform 1B Alpha Subunit or PAFAH, a 47kDa enzyme essential for precise control of mitotic spindle orientation in neuroepithelial stem cells and radial glial progenitor cells. The protein is highly conserved, with near-identity between mouse and humans. It is expressed predominantly in the brain and regulates tubulin to suppress microtubule dynamics. It participates in cytoplasmic dynein-mediated nucleokinesis, somal translocation and cell motility as well as mitosis (neurogenesis) and chromosome segregation.
Lissencephaly or ‘smooth brain’ is a brain malformation characterised by a smooth or nearly smooth cerebral surface. It has many causes, but mutations in the LIS1 gene, triggering disruption in the migration of post-mitotic neurons during gestation, causes classical lissencephaly. Symptoms might include unusual facial appearance, psychomotor retardation and seizures.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5 - 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1444 detects a band of approximately 45kDa in HeLa cell lysate.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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