AHP1421 recognises human Death Domain-Associated Protein 6, a novel Fas binding protein that has been designated DAXX.
DAXX binds specifically to the death domain of Fas and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway.
DAXX is predominantly a nuclear protein, where it acts as a repressor of EtsI and Pax3 transactivation. When in association with ASK1 kinase DAXX translocates from the nucleus to the cytoplasm.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/7000 - 1/45000
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin(1)
2.5ug/ml
Immunoprecipitation
Western Blotting
1/200 - 1/1000
(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human DAXX was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
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