AHP1396 is specific for human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor which regulates adipose-tissue mass/body weight through hypothalamic effects.
The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/16000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1396 detects a band of approximately 150kDa in human brain cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
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