AHP1394 specifically recognises human ezrin, otherwise known as villin 2, an 80kDa cytoplasmic peripheral membrane protein and member of the ezrin, radixin, moesin (ERM) protein family, which acts as protein-tyrosine kinase substrate.
Ezrin is enriched in microvilli and other cell surface structures and plays a key role in cell surface structure adhesion, membrane trafficking, motility and organization. Ezrin acts as a linker protein between the plasma membrane and the actin cytoskeleton, and provides anchorage for cell surface molecules such as ICAM and CD44. Studies have implicated ezrin in the metastatic capacity of various types of human tumours including breast, prostate, bone and colon cancers.
Species Cross-Reactivity
Target Species
Cross Reactivity
Dog
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/16,000
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1.0 - 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1394 detects a band of approximately 80kDa in human placenta and duodenum cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antiserum to human ezrin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.